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Szymon Kmiecik

is about to graduate. 🎓

Angestellt, Postdoctoral Researcher, Heidelberg University
Heidelberg, Deutschland

Fähigkeiten und Kenntnisse

Protein Expression
Protein Purification
Molecular Biology
Biotechnologie
Conjugates Chemistry
Protein Crystallization
Forschung

Werdegang

Berufserfahrung von Szymon Kmiecik

  • Bis heute 4 Jahre und 1 Monat, seit Mai 2021

    Postdoctoral Researcher

    Heidelberg University

    Duties: • Project management including multidirectional project development, • communication and projects coordination with collaborators, • literature review and writing of scientific publications, • presentation of research data during meetings and conferences, • teaching and supervision of students, • assay development and characterization.

  • 4 Jahre und 3 Monate, März 2017 - Mai 2021

    PhD Candidate

    Heidelberg University

    PhD project title: Molecular mechanisms of the mammalian heat shock transcription factor Hsf1 Duties: • protein production and purification, • protein biochemistry and analysis (FPLC, RP-HPLC, IMAC, IEC, SEC, Western blot, SDS-PAGE, BN-PAGE, EMSA, CD, stopped-flow, Fluorescence/Fluorescence polarization), • hydrogen exchange mass spectrometry (HX-MS): an investigation of conformational dynamics and mapping interaction sites of macromolecules. • literature review and scientific writing.

  • 1 Jahr und 8 Monate, Juli 2015 - Feb. 2017

    Biotechnology Specialist

    Pure Biologics Inc.

    Duties: • realization of projects related to protein production and purification, • protein analysis (SDS-PAGE, Western blot, dot-blot), • cell cultures; adherent cell culture at industrial scale, • validation documentation and validation of analytical methods, • documentation of work in the system supporting the laboratory work CS-17 LIMS, • literature review, • reports writing, • Internal Auditor associated with PN-EN ISO/IEC 17025:2005 norm.

  • 1 Jahr und 2 Monate, Juni 2014 - Juli 2015

    Research Assistant

    University of Virginia

    Duties: • protein production (optimization of expression conditions), • protein purification (affinity chromatography, gel filtration chromatography, ion-exchange chromatography), • protein analysis (SDS-PAGE, pull-down), • molecular cloning (constructs and primers design), • proteins crystallization (crystallization setup, alternative approaches for protein crystallization), • literature review and presentation of research data.

  • 1 Jahr und 5 Monate, Feb. 2013 - Juni 2014

    Master Student

    University of Wroclaw

    Synthesis, purification, and cytotoxicity assessment of affibody and auristatin (protein-drug) bioconjugates

  • 11 Monate, März 2012 - Jan. 2013

    Bachelor Student

    Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, PAS

    Synthesis, purification, and cytotoxicity assessment of methotrexate and epirubicin (drug-drug) bioconjugates

Ausbildung von Szymon Kmiecik

  • 2 Jahre und 6 Monate, Feb. 2013 - Juli 2015

    Biotechnology

    Wrocław University of Science and Technology

    Molecular biotechnology and biocatalysis

  • 3 Jahre und 5 Monate, Sep. 2009 - Jan. 2013

    Biotechnology

    Wrocław University of Science and Technology

Sprachen

  • Polnisch

    Muttersprache

  • Englisch

    Fließend

  • Deutsch

    Gut

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