Szymon Kmiecik

Duties: • Project management including multidirectional project development, • communication and projects coordination with collaborators, • literature review and writing of scientific publications, • presentation of research data during meetings and conferences, • teaching and supervision of students, • assay development and characterization.

PhD project title: Molecular mechanisms of the mammalian heat shock transcription factor Hsf1 Duties: • protein production and purification, • protein biochemistry and analysis (FPLC, RP-HPLC, IMAC, IEC, SEC, Western blot, SDS-PAGE, BN-PAGE, EMSA, CD, stopped-flow, Fluorescence/Fluorescence polarization), • hydrogen exchange mass spectrometry (HX-MS): an investigation of conformational dynamics and mapping interaction sites of macromolecules. • literature review and scientific writing.

Duties: • realization of projects related to protein production and purification, • protein analysis (SDS-PAGE, Western blot, dot-blot), • cell cultures; adherent cell culture at industrial scale, • validation documentation and validation of analytical methods, • documentation of work in the system supporting the laboratory work CS-17 LIMS, • literature review, • reports writing, • Internal Auditor associated with PN-EN ISO/IEC 17025:2005 norm.

Duties: • protein production (optimization of expression conditions), • protein purification (affinity chromatography, gel filtration chromatography, ion-exchange chromatography), • protein analysis (SDS-PAGE, pull-down), • molecular cloning (constructs and primers design), • proteins crystallization (crystallization setup, alternative approaches for protein crystallization), • literature review and presentation of research data.

Synthesis, purification, and cytotoxicity assessment of affibody and auristatin (protein-drug) bioconjugates

Synthesis, purification, and cytotoxicity assessment of methotrexate and epirubicin (drug-drug) bioconjugates

Molecular biotechnology and biocatalysis