Dr. Yan Wang-Walles

Developed a new LC-MS assay to quantify acylcarnitine and CoA esters in tissue samples for study of cancer cachexia treatment. Investigated the pharmacokinetics and metabolism of (R,R)-4-methoxyfenoterol in human intestinal and liver microsomes by LC-MS/MS. Developed a CE method to determine the ATP/ADP levels in normal and irritated mitochondria to screen cell damage caused by exposure to high dose radiation. Developed a CE/LIF assay to determine free and protein bound glutathione in HepG2 cells.

Designed and Constructed laser desorption solid Phase microexctraction (SPME) interfaces to MS, GC/GC-MS and IMS. Lead industrial collaboration with MDS Sciex to design and develop automated SPME/MALDI MS device for peptides and protein digest analysis. Performed weekly tutorials mentored and evaluated student's performance.

Developed methods for FT-IR spectrometer applications in area of industrial quality control. Interacted with customers to demonstrate new FT-IR technology and applications Organized Nicolet FT-IR theory and application workshops.

Instructed "analytical chemistry" and "chemical separation methods" courses. Conducted Laboratory demonstration for NMR spectrometer. Supervised undergraduate students for fourth year project.

Laser Desorption and MALDI, Mass spectrometry, Ion mobility, sample preparation techniques

Isolated and purified diterpenoid alkaloids from traditional Chinese herbal medicines and elucidated the structures using NMR.