madhura khare

Online tutoring is the freelancing job. I teach high school and graduate students online in Biology, Biochemistry, bioinformatics, proteomics etc.

• Amplification and Gene cloning of various H. Armigera Gut Trypsins into bacterial expression vectors like pET28a, pEGX etc. • Optimizing culture induction temperatures and Bulk expression and purification using chromatographic techniques. • Setup crystallization trials for purified protein. Gene cloning and expression, Protein Purification, Akta system: Gel filtration, Ion exchange, affinity and desalting column.

• Worked on “Profiling and elucidating the role of Exosomes in inducing aggressiveness in normal cells”. • Assisted in optimization of isolation of exosomes from spent media using gradient ultracentrifugation and immuno-precipitation on dyna beads. Cell culture, Sucrose gradient ultracentrifugation, RNA Isolation and c-DNA synthesis, Immuno-precipation.

• Record maintainance of Blood Sample (HNSCC) collected along with its meta-data. • Selected pathogenic mutations were modeled and simulated in-silico for 100ns out to see the related structural changes Gene cloning and expression, Protein Purification, Gel filtration column (S75, S200), LC MS/MS data analysis, Ammonium sulphate precipitation SQL, Biological databases and pathway analysis HTML, CSS and JavaScripts.

• Worked on “MD simulation studies of various antimicrobial peptides in TFE: Water environment” Gromacs, Unix/Linux operation, sequence analysis, remote-local server access

Biotechnology, Bioinformatics, Molecular Biology, Biochemistry

Human Anatomy, Physiology, transducers