Dr. najmeh Heusch

transformation

- Supervised bachelor, master and PhD projects and trained students in in-vitro cultures and molecular and biochemical techniques. - teaching in several areas of plant biology for undergraduates and graduates students - holding biology seminars and molecular workshops (cloning) - research in secondary metabolites pathways, especially lignan biosynthesis pathway - collaboration in different projects at IFIB (traveling to Germany)

1) Biochemical characterization of a pinoresinol lariciresinol reductase like protein: - Heterologous expression of the protein in E. coli - Purification of the protein by Ni-NTA affinity chromatography and gelfiltration - Determination of the enantiospecifiity - Determination of enzyme kinetic parameters - Protein crystallography for the determination of the 3D protein structure 1) Genetic manipulation of the lignan biosynthesis in hairy roots: - Cloning of RNAi vector constructs

Biosynthesis of lignans under coniferaldehyde and methyl jasmonate treatments in hairy roots of Linum album