Ale Mieli

Executed nucleic acid extraction and quality control workflows supporting biotechnology research projects with emphasis on reproducibility and documentation rigor. -Extracted and QC'd DNA/RNA from 200+ plant/bacterial samples using Nanodrop and gel electrophoresis, achieving 98% suitability rate for downstream PCR and NGS applications -Performed PCR and RT-qPCR genetic analysis with strict adherence to protocols; maintained detailed documentation of all experimental parameters and deviations

Designed and executed molecular cloning workflows for plant genetic engineering, with a focus on method optimization, quality validation, and reproducible protocols. -Engineered Arabidopsis lines using Gateway cloning technology; constructed plasmids and performed E.coli/Agrobacterium transformations with quality checkpoints at each stage - Maintained detailed electronic and paper lab notebooks documenting all protocols, modifications, and results for traceability

Focus: molecular biology, genetic engineering, bioinformatics, microbial/plant biotechnology.