Dr. Anna-Maria Ordelheide

* Laboratory and personnel management, Scientific writing and publishing * Research focus: insulin-desensitizing properties of saturated fatty acid-induced myokine G-CSF in humans/generation of iPSCs from urine and skin biopsies (current) * Methods: tissue culture (primary human myoblasts and preadipocytes; differentiation to myotubes and adipocytes, hiPSC), cell proliferation assays, RT-PCR, siRNA, western blot, glycogen synthesis, ELISA, bright-field microscopy

* Research focus: expression of ALDH1 in endocrine cells of Type 1 Diabetes patients * Methods: histology, immunofluorescence, confocal imaging, human islets, flow cytometry, ImageJ

* Research focus: role of NOR-1 in insulin gene regulation and insulin secretion * Methods: human and rat islets, insulin content, insulin secretion, tissue culture, western blot, chromatin immunoprecipitation, luciferase reporter gene assays, RT-PCR, lentivirus, S2, bright-field microscopy

* Member of the DFG GRK 1302: The PI3K Pathway in Tumour Growth and Diabetes * Topic: “Analysis of 14-3-3 protein binding to the kinesin KIF1Balpha” * Methods: PCR, cloning, transfection, immunoprecipitation, western blot, tissue culture, immunofluorescence, confocal imaging

Botany, Genetics, Toxicology, Organic chemistry