Dr. Aparna Viswanathan Ammanath

Project: Role of immunity related GTPases in secretory autopahgy and Salmonella Typhimurium infections. FELASA - Basic Mouse Module and Surgery Module (Mouse) certified

Development of narrow spectrum anti-infective peptides from S.aureus lipoprotein with no inflammatory properties (EP Patent EP4201483A1). Standardized protocol for antibiotics screening in Galleria mellonella larvae model. Proficient in handling of mammalian cell lines. Worked with International collaborators in successful planning, execution and analysis of experiments. Mentored Master's students, maintained records and wrote Scientific articles

• Thesis: Development of narrow spectrum anti-infective peptides from S.aureus lipoprotein with no inflammatory properties (EKUT filed a priority application with the European Patent Office, Priority date 2021-12-22). • Standardized protocol for antibiotics screening in Galleria mellonella larvae model. • Proficient in endocytosis and phagocytosis studies, wound healing, chemotaxis, immune stimulation (ELISA) and toxicity assays on mammalian cell lines (primary and immortalized).

• Project: Development of phytochemical incorporated polymer gel for infectious wound healing in mice model • Validated protocol for testing phytochemicals for topical applications in BALB/c mice. • Successfully designed polymeric nanoparticles for targeted drug delivery. • Well versed in microbe-eukaryotic cell co-culture, nanoparticle uptake quantification by flow cytometry, neutrophil & RNA isolation and PCR.

Mice handling and Surgery