Flavio Schwald

- Develop advanced hepatic in vitro models for mechanistic safety assessment and DILI risk evaluation. - Design cell-based assays using viability, functionality and high-content imaging readouts. - Support portfolio projects through experimental data generation, troubleshooting and mechanistic interpretation. - Contribute to 3R-relevant assay strategies, model miniaturization and benchmarking of complex liver models.

- Applied complex hepatic in vitro systems for preclinical safety assessment. - Generated cytotoxicity and functionality data to support DILI risk evaluation. - Optimized staining, imaging and high-content analysis workflows for improved assay robustness. - Supported 3R and innovation projects focused on advanced liver model development and benchmarking. - Collaborated cross-functionally with project teams, analytical experts and external partners.

- Developed and characterized transfected cell models for assay development. - Established cell-based assays for compound screening in an HTS environment. - Measured ion channel activity using calcium flux-based assay platforms. - Worked with FDSS7000EX and FLIPR Tetra systems for functional readouts.

- Worked with primary cells and hiPSC-based models across preclinical research areas. - Developed and applied cell-based assays using differentiated hiPSC-derived models. - Performed quantitative high-content imaging on live and fixed cells. - Conducted protein analysis using BCA assay, SDS-PAGE and Western blot.

- Completed a biology laboratory technician apprenticeship with rotations in DMPK/NPK-PD, PCS and Oncology. - Built a broad technical foundation in in vivo work, autoradiography, cell culture, FACS and ELISA-based assays. - Gained hands-on experience in primary cell isolation, immunostaining and preclinical laboratory workflows.

- Focused on assay development, analytical technologies, molecular biology and genetics. - Conducted project work on 3D liver microtissues and flow cytometry-related sample preparation. - Completed bachelor thesis work on the development of an in vitro blood-brain barrier model.

- Built a practical and theoretical foundation in life sciences. - Gained broad exposure to professional laboratory work in biology. - Developed core hands-on laboratory and documentation skills.