Katharina Theinert

Design, performance and documentation of development work for the expression of recombinant proteins in human cell lines (HEK293F, GEX) Development and optimization of perfusion processes in small scale (1L-5L) in various bioreactor systems (Dasgip, Finesse, GE, Infors) Assay development and optimization (chromogenic assay, binding assay) Electronic Lab Notebook (ELN) Key User function (IDBS) LIMS Key User function

Establishment of a flow chamber system to investigate blood coagulation factor XII Fluorescence and confocal microscopy Assays to investigate blood coagulation: ETP, ball coagulometer, flow chamber Purification of antibodies via affinity chromatography Characterization of purified antibodies by SDS-PAGE and Western Blot Participation in animal studies (mice, rabbit) Technical assistance: Larsson et al. (2014) Cell lines: HEK293, hybridoma

Investigation of the influence of secondary metabolites (isolated from different fungi strains) on certain pathways linked to inflammatory bowel disease -transcriptional level: reporter gene assays and quantitative real-time PCR -translational level: proteome profiling Cell lines: E. coli, Colo 320 (human adenocarcinoma), DLD-1 (human adenocarcinoma))

Development of an ELISA to determine antibody titers against blood group A and B antigens Development of an immuno-adsorption column for extra-corporeal removal of antibodies directed against blood group A or B antigens see also: L. Lindberg, K. Theinert, J. Liu, J. Holgersson (2012) Adsorption of chain type–specific ABO antibodies on Sepharose-linked A and B tetrasaccharides. Transfusion

Microbiological Quality Control: Set-up of an electronic archiv for microbiological test records Participation in additional trainings: clean room class D and C, GLP, GMP

Biomedical Science and Technology