Thomas Winogrodzki

Gene therapy of retinal diseases

"The generation and characterization of a physiologically relevant porcine model of inflammatory bowel disease" - I honed diverse skills, from genetic modifications and organoid cultures to data management. My research included the generation of the first genetically modified pig model for Crohn’s Disease-like ileocolitis, analysis of polyp development in a porcine model of FAP, and humanization of the pig NTCP gene in somatic cells, alongside enhancing HDR-efficiency via RNA aptamers.

M.Sc. Molekulare Biotechnologie; "Optimisation of gene-targeting by CRISPR/Cas9 using porcine somatic cells"

In this role, I was responsible for the execution of already established and novel methods to investigate the role of Mt-UPR in chronic intestinal diseases. My tasks included the establishment, execution and analysis of standard molecular biological methods, the cultivation of ex vivo enteroids, histological work and the elaboration of my findings in scientific documents.

In this role, I was responsible for the efficient and accurate genotyping of novel mouse lines and participated in the development of breeding plans. Additionally, I assisted in live cell imaging of neonatal motoneurons.

In this internship, I participated in increasing secretion volumes of biotechnologically relevant proteins in B. subtilis production strains by genetic engineering. Additionally, I discussed current successes and problems with the team through regular team reports.

B. Sc. Molekulare Biotechnologie; "Characterization of co-receptors in Populus"

I independently executed multiple research projects in translational science. My tasks involved intense literature reviewing, genetic engineering, molecular biology methods, histology, 2/3D cell cultures, and immune cell-organoid co-cultures, confocal microscopy, next generation sequencing, and data analysis. My work extended to embryology and animal studies, the preparation and publication of scientific documents and mentoring of junior scientists during theses and hands-on courses.

Molekulare Aspekte der Biotechnologie mit den fünf Säulen: Zelle, Technik, Biomoleküle, Medizin und Organismen