Ivanéia Nunes

• Served as in-house ELISA subject matter expert for RNA immunogenicity assays in primary human monocytes, optimizing stimulus concentration ranges and dilution parameters, eliminating the need for serial titration in each experiment. • Delivered hands-on technical training and troubleshooting support for 20+ users on monocyte-based assay platforms (isolation, flow cytometry, ELISA, data analysis), enabling researchers to independently run experiments and generate reproducible data. • Established and tr

The PhD project was initiated at TU Dresden and continued at Heidelberg University after the principal investigator’s appointment there, ensuring continuity of scientific direction while adapting to a new institutional environment.

• Co-created and delivered technical content (infographics, webinars, and live sessions) on laboratory techniques, experimental design, and troubleshooting for a community of 11,000+ life science professionals. • Translated complex bench-level methods into accessible, practical resources from assay optimization to data interpretation, demonstrating the ability to communicate technical concepts clearly to diverse scientific audiences.

• Designed novel ELISA protocols to quantify IgE, IgG, and IgA in murine serum from allergic inflammation, establishing a standardized workflow that was successfully adopted by lab members. • Isolated and restimulated immune cells from mesenteric lymph nodes and spleen across 2 distinct preclinical models, contributing to mucosal and systemic immune response characterization. • Performed end-to-end histological characterization of intestinal tissues (HE, PAS staining, morphometry via ImageJ), validating a

• Expressed and purified recombinant protein variants for Staphylococcus aureus vaccine candidates; supported early citoxicity assessmen in human neutrophils (HL-60 cells). • Engineered a toxin knockout strain of Staohylococcus aureus used in downstream preclinical infection models. • Contributed to experimental design and systematic troubleshooting, including the optimization of conditions for recombinant protein expression and purification, through data analysis and regular scientific discussions.

Thesis title: Ribonuclease 6 processes bacterial RNA into agonists for TLR8 but is impaired by 2’-O-methyl-uridine

Thesis title: Development of an experimental model of shrimp allergy.

Sponsored by the Brazilian Mobility Scientific Program (BSMP) and the International Institute of Education (IIE). Attended the Intensive English Program, followed by undergraduate coursework.

Thesis title: Characterization of the cellular infiltrate on the skin of mice immunized against Staphylococcus aureus in an air pouch model.