soroush azarian

Led 5 L bioreactor fermentation of antimicrobial peptide (darobactin B) Production. Developed and executed upstream bioprocessing strategies using recombinant E. coli. Controlled and optimized bioreactor performance to support secondary metabolite production by identification and resolving common bioreactor operational challenges during cultivation. Performed large-scale isolation and purification of Darobactin B. Analyzed product formation using LC-MS and, achieved analytical-scale purification of Darobac

• Performed large-scale cultivation (40 L total volume) of E. coli in shaking flasks for darobactin production • Conducted adsorption-based product recovery using Amberlite resin for darobactin separation • Extracted RNA and DNA, and performed PCR amplification

• Performed isolation and confirmation of pure bacterial culture from clinical specimens • Identified MRSA strains using selective media and antibiotic susceptibility testing • Detected ESBL-producing Gram-negative organisms through screening and confirmatory tests. • Conducted identification of Gram-negative bacteria, including Brucella spp.

·       Managed full B2B sales cycle for international pharmaceutical accounts, from lead qualification and proposal preparation through to order fulfillment and invoicing ·       Developed and maintained long-term customer relationships across global accounts, driving repeat business and customer retention ·       Prepared quotations, technical proposals, and pricing documentation for international clients ·       Coordinated product communication, order management, and delivery tracking across multiple