prathamesh thakare

Conducted Master's thesis research in Bioprocess Engineering focused on the development of a growth-coupled Escherichia coli platform for screening glycolaldehyde synthase enzymes. • Engineered and characterized E. coli strains for a pyridoxine-auxotrophy-based enzyme screening platform. • Performed molecular biology and microbial cultivation workflows including strain construction, gene-deletion verification, colony PCR, growth characterization and experimental optimization. • Investigated metabolic engine

Founded and led a life-science education and training organization, combining scientific communication with entrepreneurship, team leadership and operational management. • Built and managed biotechnology-focused academic and professional training programmes reaching more than 500 students. • Recruited, coordinated and mentored a multidisciplinary faculty team while maintaining programme quality and delivery standards. • Managed budgeting, resource allocation, client acquisition, marketing and day-to-day org

Worked within the Downstream Processing (DSP) team at Wockhardt Research & Development Centre on the purification and process development of therapeutic proteins. • Supported downstream processing workflows involving chromatographic purification, HPLC, centrifugation, spectroscopy and buffer preparation. • Worked with pilot- and industrial-scale membrane and cassette filtration systems used in biopharmaceutical processing. • Evaluated the binding capacity and performance of chromatography resins used for mo