Dr. Jashwant Kumar

Upstream process development for Biologics

Upstream Cell Culture Process Development for monoclonal antibody biosimilars: - Early stage process development studies (clone selection, media feeds screening, physical paramters optimization, bioreactor suitability and pilot scale consistency runs) for mAb biosimilar - Scale-up of mAb processes to 2kL Single Use Bioreactors (Constant kLa, P/V approach based upstream prcocess scale-up) - Technology Transfer (Process TTDs preparation) - Process productivity Improvement -Concentrated Fed-Batch Process

Upstream Cell Culture Process Development: Responsible for Late Stage -Upstream Cell Culture Process Development Studies for monoclonal antibody biosimilar development ( Risk assessment by FMEA, Scale Down Model Development and Process Chracterization Studies). Additionally - Upstream Gap Experiments, Pilot plant and Manufacturing Support, Troubleshoot issues related to cell culture, Utilization of statistical methods and design of experiments to drive process improvements and Support Regulatory Filing

Process Development Scientist, Cell Culture Process: -Hands on experience with cell culture techniques and aseptic handling -Experience in inoculum propagation of mammalian cell line in shake flask and seed bioreactor -Experience in operation of lab scale (1 L), high-throughput mini-bioreactor (Ambr250) and operation of 1L bioreactor perfusion cell culture using ATF2

Production of recombinant lipase GTL from Corynebacterium glutamicum and product purification with Immobilized Metal Ion Affinity Chromatography

Helped research scholar in their project work - Plant tissue culture (Vigna mungo), DNA isolation from plant leaves, Setting PCR reactions

Bachelor Thesis- Parameter optimization for protease production

Helped research scholars in thier projects works- DNA isolation from Human Blood, Setting PCR reactions, Electrophoresis

Pharmaceutical Biochemical Engineering, Cell Culture Techniques, Protein Purification Techniques, Bioprocess Automation, Off-Line & At-Line Analytics, Good Manufacturing Practice, Pharmacology, Analysis, Modeling and Simulation of Bioprocesses, Biochemical Analytics

Biochemistry, Cell Biology, Microbiology, Principle of Chemical Engineering, Molecular Genetics, Bioprocess Engineering, Downstream Processing, Medical and Pharmaceutical Biotechnology, Protein Engineering, Immunology, Genetic Engineering and Bioethics