Dr. Prasad Kakarla

• Purification of monoclonal antibodies (mAb). • Modeling and optimization of protein purification process. • Surface characterization of various chromatographic adsorbent and biomass types. • Evaluation of peptide distribution profiles in various food samples.

• Physico chemical, biochemical characterization and stability studies of therapeutic proteins. • Failure investigation and discrepancy management. • Troubleshooting, periodic calibration and maintenance of HPLC. • Prepared SOPs & STPs.

• Set up laboratory for mammalian cell culture. • Developed a method for Calcium influx assay in Jurkat and Rat Basophilic Leukemia (RBL) cells. • Established a method to detect the inhibitory effect of drug candidates on cytokine production in whole blood and peripheral blood mono-nucleated cells (PBMC). • High throughput assays (enzymatic and cell based) to estimate the inhibitory effect of drug candidates on the purified enzyme and on the growth of high c- Met expressing sk-LMS-1 cell line

• Validated colon adenocarcinoma cell (Caco-2) permeability assay to circumvent the bioavailability and bioequivalence (BA/BE) studies. • Optimized cytotoxicity assay in various mammalian cell lines, to determine IC50 of drug candidates. • Developed HPLC methods to quantify various potential pharmaceutical molecules in biological samples. • Prepared project plan and cost estimates for research proposals.